Identification and drug susceptibility testing of Mycobacterium thermoresistibile and Mycobacterium elephantis isolated from a cow with mastitis.
10.3760/cma.j.issn.0254-6450.2018.05.025
- Author:
W B LI
1
;
L Y JI
2
,
3
;
D L XU
4
;
H C LIU
4
;
X Q ZHAO
4
;
Y M WU
1
;
K L WAN
4
Author Information
1. Institution of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, China.
2. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
3. Department of Laboratory Medicine, Shanghai East Hospital, Tongji University, Shanghai 200123, China.
4. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
- Publication Type:Journal Article
- Keywords:
Cow with mastitis;
Mycobacterium elephantis;
Mycobacterium thermoresistibile;
Non-tuberculosis mycobacterium
- MeSH:
Animals;
Anti-Bacterial Agents/pharmacology*;
Antitubercular Agents/pharmacology*;
Cattle;
Drug Resistance, Bacterial;
Female;
Humans;
Mastitis, Bovine/microbiology*;
Microbial Sensitivity Tests;
Milk/microbiology*;
Mycobacterium/isolation & purification*;
Mycobacterium Infections/veterinary*;
Mycobacterium tuberculosis/drug effects*;
Nontuberculous Mycobacteria/isolation & purification*;
Polymerase Chain Reaction;
RNA, Ribosomal, 16S/genetics*
- From:
Chinese Journal of Epidemiology
2018;39(5):669-672
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To understand the etiological characteristics and drug susceptibility of Mycobacterium thermoresistibile and Mycobacterium elephantis isolated from a cow with mastitis and provide evidence for the prevention and control of infectious mastitis in cows. Methods: The milk sample was collected from a cow with mastitis, which was pretreated with 4% NaOH and inoculated with L-J medium for Mycobacterium isolation. The positive cultures were initially identified by acid-fast staining and multi-loci PCR, then Mycobacterium species was identified by the multiple loci sequence analysis (MLSA) with 16S rRNA, hsp65, ITS and SodA genes. The drug sensitivity of the isolates to 27 antibiotics was tested by alamar blue assay. Results: Two anti-acid stain positive strains were isolated from the milk of a cow with mastitis, which were identified as non-tuberculosis mycobacterium by multi-loci PCR, and multi-loci nucleic acid sequence analysis indicated that one strain was Mycobacterium thermoresistibile and another one was Mycobacterium elephantis. The results of the drug susceptibility test showed that the two strains were resistant to most antibiotics, including rifampicin and isoniazid, but they were sensitive to amikacin, moxifloxacin, levofloxacin, ethambutol, streptomycin, tobramycin, ciprofloxacin and linezolid. Conclusions:Mycobacterium thermoresistibile and Mycobacterium elephantis were isolated in a cow with mastitis and the drug susceptibility spectrum of the pathogens were unique. The results of the study can be used as reference for the prevention and control the infection in cows.
