Transmitted drug resistance among HIV infected men who have sex with men in Tianjin, 2014-2017.
10.3760/cma.j.issn.0254-6450.2018.05.015
- Author:
M N ZHENG
1
;
T L NING
;
N ZHOU
;
X ZHAO
;
L LI
;
J J ZHU
;
S H CHENG
Author Information
1. Department for AIDS/STD Control and Prevention, Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China.
- Publication Type:Journal Article
- Keywords:
HIV;
Men have sex with men;
Transmitted drug resistance
- MeSH:
China;
Drug Resistance, Viral/genetics*;
Genes, pol;
Genotype;
HIV Infections/transmission*;
HIV Reverse Transcriptase/genetics*;
HIV Seropositivity/genetics*;
HIV-1/isolation & purification*;
Homosexuality, Male/statistics & numerical data*;
Humans;
Male;
Mutation;
Phylogeny;
Polymerase Chain Reaction;
Prevalence;
RNA, Viral/genetics*;
pol Gene Products, Human Immunodeficiency Virus/genetics*
- From:
Chinese Journal of Epidemiology
2018;39(5):619-624
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To understand prevalence and transmission of transmitted drug resistance (TDR) among HIV infected men who have sex with men (MSM) in Tianjin from 2014 to 2017. Methods: A total of 225 blood samples were collected from HIV infected MSM in Tianjin from 2014 to 2017. Pol gene fragments were obtained by viral RNA extraction and nested PCR amplification. Phylogenetic and drug resistance analyses were conducted. Results: A total of 205 samples were successfully sequenced and analyzed. Based on pol sequences, 53.2% (109/205), 28.8% (59/205), 10.2% (21/205), 4.9% (10/205) and 2.9% (6/205) of the samples were positive for HIV subtypes CRF01_AE, CRF07_BC, B, CRF55_01B and unique recombinant forms (URFs). Twenty transmission clusters, including 75 sequences, were identified and 62.5% (10/16) of sequences with TDR were in 5 clusters. The prevalence of TDR was 7.8% between 2014 and 2017. The annual prevalence rate increased from 3.9% (2/51) in 2014, 5.7% (3/53) in 2015, 9.6% (5/52) in 2016 to 12.2%(6/49) in 2017, the difference was not significant (χ(2)=2.504, P=0.127). CRF01_AE and B strains had high TDR prevalence (3.4%, 7/205) and (2.9%, 6/205), respectively. The TDR mutation was mainly NNRTIs, the TDR prevalence was 6.3% (13/205). In contract, the TDR prevalence of NRTIs and PIs were 1.5% (3/205) and 1.0% (2/205) respectively. Conclusion: Results from this study suggested that the prevalence of HIV-1 TDR strains in MSM was serious in Tianjin. It is necessary to take effective prevention and control measures.
