Association between obesity and DNA methylation among the 7-16 year-old twins.
10.3760/cma.j.issn.0254-6450.2018.04.011
- VernacularTitle:7～16岁儿童青少年肥胖与DNA甲基化相关性的双生子研究
- Author:
C X LI
1
;
Y GAO
1
;
W J GAO
1
;
C Q YU
1
;
J LYU
1
;
R R LYU
2
;
J L DUAN
2
;
Y SUN
2
;
X H GUO
3
;
S F WANG
1
;
B ZHOU
1
;
G WANG
2
;
W H CAO
1
;
L M LI
1
Author Information
1. Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing 100191, China.
2. Beijing Center for Disease Control and Prevention, Beijing 100013, China.
3. Chaoyang District Center for Disease Control and Prevention, Beijing 100021, China.
- Publication Type:Twin Study
- Keywords:
Children and adolescents;
Epigenetics;
Genome-wide DNA Methylation;
Obesity;
Twins
- MeSH:
Adolescent;
Bayes Theorem;
Beijing;
Body Weight;
Child;
DNA Methylation/genetics*;
Epigenesis, Genetic;
Female;
Genome-Wide Association Study;
Humans;
Male;
Obesity/genetics*;
Twins, Monozygotic;
Waist Circumference
- From:
Chinese Journal of Epidemiology
2018;39(4):443-448
- CountryChina
- Language:Chinese
-
Abstract:
Objective: On whole-genome scale, we tried to explore the correlation between obesity-related traits and DNA methylation sites, based on discordant monozygotic twin pairs. Methods: A total of 90 pairs of 6-17 year-old twins were recruited in Chaoyang district, Yanqing district and Fangshan district in Beijing in 2016. Information on twins was gathered through a self-designed questionnaire and results: from physical examination, including height, weight and waist circumference of the subjects under study. DNA methylation detection was chosen on the Illumina Human Methylation EPIC BeadChip. R 3.3.1 language was used to read the DNA methylation signal under quality control on samples and probes. Ebayes function of empirical Bayes paired moderated t-test was used to identify the differential methylated CpG sites (DMCs). VarFit function of empirical Bayes paired moderated Levene test was used to identify the differentially variables CpG sits (DVCs) in obese and normal groups. Results According to the obesity discordance criteria, we collected 23 pairs of twins (age range 7 to 16 years), including 12 male pairs. A total of 817 471 qualified CpG loci were included in the genome-wide correlation analysis. According to the significance level of FDR set as <0.05, no positive sites would meet this standard. When DMC CpG site cg05684382, with the smallest P value (1.26E-06) as on chromosome 12, the DVC CpG site cg26188191 with the smallest P value (6.44E-06) appeared in CMIP gene on chromosome 16. Conclusions: In this study, we analyzed the genome-wide DNA methylation and its correlation with obesity traits. After multiple testing corrections, no positive sites were found to have associated with obesity. However, results from the correlation analysis demonstrated sites cg05684382 (chr: 12) and cg26188191 (chr: 16) might have played a role in the development of obesity. This study provides a methodologic reference for the studies on discordance twins related problems.
