Association between expression of plasma miRNA and the risk of childhood acute lymphocytic leukemia
10.3760/cma.j.issn.0254-6450.2017.09.022
- VernacularTitle:血浆miRNA表达与儿童急性淋巴细胞白血病风险的关联研究
- Author:
Dongmei YUAN
1
,
2
;
Siying WU
;
Suli HUANG
;
Weichao JIANG
;
Yuebin KE
Author Information
1. 518055 深圳市疾病预防控制中心医学分子生物学重点实验室
2. 350108福州,福建医科大学公共卫生学院流行病与卫生统计学系
- Keywords:
Childhood acute lymphocytic leukemia;
Biomarker
- From:
Chinese Journal of Epidemiology
2017;38(9):1252-1258
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the characteristics of distribution and expression profiles of plasma miRNA in childhood acute lymphocytic leukemia (cALL) patients;the association between cALL incidence risk and plasma miRNA levels;the feasibility of plasma miRNA serving as cALL diagnostic biomarker.Methods A total of 111 pairs of newly diagnosed cALL patients and patients with fractures were collected from Shenzhen Children's Hospital,China,between January 2015 and November 2016.Age and sex of the cases and controls were 1 ∶ 1 matched and LNATM miRNA microarray was performed using 4 pairs of cALL and controls selected from the sample population.The expression level of miRNA was validated by real time quantitative PCR.Conditional logistic regression analysis was applied to evaluate the association between miRNA expression levels and the incidence risk of cALL.The receiver operating characteristic curve (ROC) and reclassification analysis were conducted to assess the feasibility of miRNAs serving as biomarkers for cALL.Results A total of 204 differentially expressed miRNA were screened out and let-7f-5p,miR-5100,miR-25-3p and miR-3654 were selected for validation identified according to the inclusion criteria.The expression levels of let-7f-5p,miR-5100 and miR-25-3p in the cALL patients were significantly lower than those of the controls (P＜0.01).After adjusting for confounding factors,3 miRNAs remained significantly associated with the risk of cALL (OR and 95％CI were 0.84 (0.76-0.92),0.81 (0.73-0.90)and 0.81 (0.74-0.89),respectively.Results from both the ROC analysis and reclassification analysis showed that introduction of one or more miRNA to traditional risk factors improved the area under the curve (P＜0.05) and provided additional values to diagnosis (P＜0.01).Conclusion The expression levels of let-7f-5p,miR-5100 and miR-25-3p were significantly associated with the incidence rate of cALL,and these miRNAs might serve as promising biomarkers for cALL.
