Effects of Src on cervical cancer cells proliferation and apoptosis through ERK signal transduction pathway
10.3760/cma.j.issn.0254-6450.2017.09.021
- VernacularTitle:Src介导ERK信号通路对宫颈癌细胞增殖凋亡的作用
- Author:
Zhichao SONG
1
;
Ling DING
;
Zhiying REN
;
Xuesong SUN
;
Qian YANG
;
Lu WANG
;
Meijuan FENG
;
Chunliang LIU
;
Jintao WANG
Author Information
1. 山西医科大学公共卫生学院流行病学教研室
- Keywords:
Cervical cancer cell;
Src;
ERK signal transduction;
HPV
- From:
Chinese Journal of Epidemiology
2017;38(9):1246-1251
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Src on cervical cancer cells through ERK signal transduction pathway.Methods Experimental study was carried out in vitro.Cervical cancer cell lines Hela (HPV-positive) and C33A (HPV-negative) were treated with Src kinase inhibitor PP2.Then,the cell cycle and apoptosis of each group were evaluated by using flow cytometry (FCM).Western blotting and Real-time PCR were used to detect the levels of the expression of ERK 1/2,c-Fos and c-Jun mRNA and protein respectively.The database was established and analyzed with SPSS statistical software (version 20.0).Results After down-regulating Src,the cell proliferation was inhibited and cell apoptosis was induced.The proportions of G0/G 1 stage of Hela and C33A cell in cell cycle increased while G2/M and S stages decreased.Meanwhile,the mRNA levels of ERK 1,ERK 2,c-Fos and c-Jun increased.And the expression levels of ERK 1/2,phosphorylated ERK 1/2 (p-ERK 1/2)and phosphorylated c-Fos (p-c-Fos) protein decreased,while c-Jun and phosphorylated c-Jun (p-c-Jun)protein expression increased.In addtion,the change level of Hela cell,p-ERK 1/2 and c-Fos protein were lower than that of C33A cell before and after the Src inhibition.Conclusions Src,involved in regulating the expression of key factors of the ERK signal transduction pathway including p-ERK 1/2 and p-c-Fos,might be capable of promoting the proliferation of cervical cancer cells and inhibiting their apoptosis.The infection with HPV might have adjustable effect on this process.
