Association between phage-mediated shiga toxin and molecular distribution of CRISPR in Escherichia coli O26 : H11 or NM
10.3760/cma.j.issn.0254-6450.2017.07.019
- VernacularTitle:O26∶H11及NM大肠埃希菌CRISPR的分子分布特征及其与stx噬菌体的关系
- Author:
Jinzhao LONG
1
;
Yake XU
;
Guangcai DUAN
;
Wenjuan LIANG
;
Huiying LIU
;
Shuaiyin CHEN
;
Yuanlin XI
;
Pengfei WANG
;
Yingfang WANG
Author Information
1. 450001,郑州大学公共卫生学院
- Keywords:
Escherichia coli;
Clustered regularly interspaced short palindromic repeat;
Stx phage
- From:
Chinese Journal of Epidemiology
2017;38(7):944-949
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between phage-mediated shiga toxin and molecular distribution of CRISPR in Escherichia (E.) coli O26:H11 or NM.Methods A total of 135 E.coli O26:H11 or NM strains were collected from NCBI database.Software CRT and CRISPR Finder were used to extract CRISPR and Excel was used to assign the spacer of unique number and type CRISPR.And the relationship between CRISPR and stx phage was analyzed.Results All the 135 E.coli O26:H11 or NM strains had the CRISPR.For CRISPRI,CRISPR2.1,CRISPR2.2 and CRISPR3-4,19,22,1 and 1 subtypes were found,respectively.According to the four CRISPR sites,the strains could be divided into 40 subtypes.Stx-phage was only observed in the group C of CRISPR.Compared with E.coli of stx-phage negative,E.coli with stx-phage harbored more spacers.Conclusions CRISPR loci was extensively existed in E.coli O26:H11 or NM,and many subtypes were found in these strains.The presence of stx-phage was related to the molecular distribution of CRISPR in E.coli O26:H11 or NM.CRISPR might be a valuable biomarker to identify strains with high virulent potential.
