Genomic analysis of Wenzhou virus in rodents from Zhejiang province
10.3760/cma.j.issn.0254-6450.2017.03.022
- VernacularTitle:浙江省啮齿动物中温州病毒的基因组学分析
- Author:
Kun LI
1
;
Xiandan LIN
;
Minghui LI
;
Miaoruo WANG
;
Xiaoyu SUN
;
Yongzhen ZHANG
Author Information
1. 中国疾病预防控制中心传染病预防控制所人兽共患病室
- Keywords:
Wenzhou virus;
Rodent;
Novel variant;
Respiratory disease
- From:
Chinese Journal of Epidemiology
2017;38(3):384-387
- CountryChina
- Language:Chinese
-
Abstract:
Objective Arenavirus is a negative single-stranded RNA virus and an important human pathogen,mainly harbored and transmitted by rodents,causing severe diseases,including hemorrhagic fever and encephalitis.Following the discovery of a novel pathogenic arenavirus (Wenzhou virus,WENV),the prevalence of WENV in local small rodents was investigated.Methods By using RT-PCR,WENV was screened in 48 and 156 rodents sampled from Wenzhou and Longquan,respectively.Results Consequently,WENV was detected in 5 (10.41％) rodents sampled from Wenzhou.However,no WENV was identified in all the rodents sampled from Longquan.Genetic analysis of complete genome sequences indicated that 4 of 5 virus strains were closely related to the known Wenzhou viruses with high homology.Especially,the L and S segments of Wencheng-Rn-288 strain shared homology of 87.5％ and 91.6％ with other viruses,respectively.They formed a distinct lineage,suggesting that this strain might be a novel variant of WENV.Conclusions Our results indicate that WENV has a high prevalence and high genetic diversity among rodents in Wenzhou.As the respiratory disease caused by WENV has been detected in Cambodia,it is necessary to strengthen the surveillance for WENV in China.
