Application of whole genome sequencing technology in the epidemiology of tuberculosis
10.3760/cma.j.issn.0254-6450.2016.12.017
- VernacularTitle:全基因组测序技术在结核病流行病学调查中的应用
- Author:
Jie WU
1
;
Lihong TANG
;
Chongguang YANG
;
Huiqin YAN
;
Hua SUN
;
Xin SHEN
Author Information
1. 200336,上海市疾病预防控制中心
- Keywords:
Tuberculosis;
Multidrug-resistance;
Whole-genome sequencing
- From:
Chinese Journal of Epidemiology
2016;37(12):1644-1646
- CountryChina
- Language:Chinese
-
Abstract:
Objective To delineate the application of whole genome sequencing technology in the epidemiology of tuberculosis.Methods From 2009 to 2012,nine Mycobacterium tuberculosis that sharing identical variable number of tandem repeats genotype (VNTR) patterns were reported from two TB cases designated hospitals.Both whole-genome sequencing analysis (WGS) and epidemiologic investigations were performed to describe the transmission patterns of these Mycobacterium tuberculosis.Results By WGS analysis,two genomic clusters including 7 and 2 Mycobacterium tuberculosis were noticed,respectively.The cluster of 2 cases possessed more than 15 single nucleotide polymorphisms (SNPs) when compared to the cluster of 7 cases and suggesting that the transmission route was independent.The transmission chain based on the SNPs difference showed the process of the propagation direction and the accumulation of drug resistance mutations in each cluster.Conclusion Using a WGS-based genomic epidemiologic approach,we were able to reconstruct the tuberculosis transmission network,tracing the putative source of the transmission and determining the transmission direction or the missing links.
