Relationship between hepatitis B virus genotype,BCP/Pre-C region mutations and risk of ;hepatocellular carcinoma in Guangxi Zhuang Autonomous Region
10.3760/cma.j.issn.0254-6450.2015.07.013
- VernacularTitle:广西壮族自治区肝癌高发区HBV基因型、BCP/前C区突变与肝癌相关性的研究
- Author:
Dan ZHENG
1
;
Wei DENG
;
Tianren HUANG
;
Xiliang LI
;
Zhaofa LI
Author Information
1. 广西医科大学
- Keywords:
Hepatocellular carcinoma;
Hepatitis B virus;
Genotype;
Mutation
- From:
Chinese Journal of Epidemiology
2015;(7):725-729
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between hepatitis B virus (HBV) genotype,the mutation in basic core promoter(BCP)region/pre-core(Pre-C)region and the incidence of hepatocellular carcinoma(HCC) in Fusui county of Guangxi Zhuang Autonomous Region (Guangxi),a area with high incidence of HCC. Methods In this case-control study,53 HCC patients and 70 asymptomatic HBV carriers were enrolled. Blood samples were collected from them for serum separation and HBV DNA extraction. The DNA sequences of the S region and BCP/Pre-C region of HBV was determined by direct sequencing following nested-PCR amplification. The relationship between the genotype,gene mutation of HBV and the incidence of HCC was analyzed. Results The mutation rates of the A1762T/G1764A in the BCP region and the T1858C in the Pre-C region of HBV were significantly higher in HCC group than in control group(94.3%vs. 75.7%,P=0.006;50.9%vs. 31.4%,P=0.029). The mutation rate of A1775G was significantly higher in control group (28.6%) than in HCC group (13.2%)(P=0.041). Multiple logistic regression analysis indicated that A1762T/G1764A and T1858C mutations are the risk factors for the development of HCC (OR=5.459,95%CI:1.397- 21.332,P=0.015;OR=3.881,95%CI:1.462-10.305,P=0.006). A1775G is the protective factor in the development of HCC(OR=0.192,95%CI:0.059-0.622,P=0.006). Conclusion The present investigation showed that BCP A1762T/G1764A,A1775G and Pre-C T1858C mutations are correlated with the incidence of HCC in Fusui county of Guangxi.
