Study on the genotype and epidemic characteristics of diarrheagenic Escherichia coli isolated from diarrheal patients in Beijing
10.3760/cma.j.issn.0254-6450.2014.10.010
- VernacularTitle:北京地区腹泻病患者致泻性大肠埃希菌感染类型及其流行特征
- Author:
Mei QU
1
;
Xin ZHANG
;
Haikun QIAN
;
Bing LYU
;
Ying HUANG
;
Hanqiu YAN
;
Zhichao LIANG
;
Lei JIA
;
Quanyi WANG
Author Information
1. 100013,北京市疾病预防控制中心食物中毒诊断溯源技术北京市重点实验室
- Keywords:
Diarrheagenic Escherichia coli;
Virulence gene;
Real-time PCR
- From:
Chinese Journal of Epidemiology
2014;(10):1123-1126
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the distribution of virulence gene and the epidemiological characteristics of diarrheagenic Escherichia(E.) coli (DEC) from diarrheal patients in Beijing. Methods Stool specimens from diarrheal patients were cultured which were collected from the hospitals under sentinel surveillance program,during 2012-2013. DNA was examined by real-time PCR. Results 253 out of 6 370 specimens were positive for DEC detection with the rate as 4.0%. A total number of 262 DEC strains were isolated. Two different pathotypes of DEC strains with mixed infection,were isolated from 9 specimens. Different pathotypes would show the following profiles:42.8% for enteropathogenic E. coli (EPEC) including 42.0% atypical and 0.8% typical;38.9% for enterotoxigenic E. coli(ETEC)including 24.8%st positive,9.9%lt positive and 4.2%st and lt both positive;15.3% for enteroaggregative E. coli(EAEC);2.7% for enteroinvasive E. coli(EIEC);one strain STEC with serotype O26 ∶ K60. ETEC had obvious characteristics on age. All kinds of DEC were isolated throughout the year with seasonal fluctuation. Conclusion DEC isolates from diarrheal patients in Beijing were dominated by EPEC and ETEC,with atypical ones accounted for the majority of EPEC. One specimen was found under mixed infection. Pathotypes DEC were found to have different age and seasonal distributions.
