Etiological and molecular characteristics of diarrhea caused Proteus mirabilis
10.3760/cma.j.issn.0254-6450.2014.06.024
- VernacularTitle:致腹泻奇异变形杆菌病原学及其分子特征研究
- Author:
Xiaolu SHI
1
;
Qinghua HU
;
Yiman LIN
;
Yaqun QIU
;
Yinghui LI
;
Min JIANG
;
Qiongcheng CHEN
Author Information
1. 518055,深圳市疾病预防控制中心 深圳市科创委重大传染病监控重点实验室 深圳市病原微生物资源库建立与应用公共服务平台
- Keywords:
Proteus mirabilis;
Virulence gene;
Plasmid;
Drug-resistant
- From:
Chinese Journal of Epidemiology
2014;(6):724-728
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the etiological characteristics,virulence genes and plasmids that carrying diarrhea-causing Proteus mirabilis and to assess their relationship with drug resistance and pathogenicity. Methods Proteus mirabilis coming from six different sources (food poisoning,external environment and healthy people) were analyzed biochemically,on related susceptibility and pulsed-field gel electrophoresis (PFGE). Virulence genes were detected by PCR. Plasmids were extracted and sequenced after gel electrophoresis purification. Results The biochemical characteristics of Proteus mirabilis from different sources seemed basically the same,and each of them showed having common virulence genes,as ureC,rsmA,hpmA and zapA. However,the PFGE patterns and susceptibility of these strains were different,so as the plasmids that they carried. Plasmid that presented in the sequenced strain showed that the 2 683 bp length plasmid encodes qnrD gene was associated with the quinolone resistance. Conclusion Etiological characteristics and molecular characteristics of Proteus mirabilis gathered from different sources,were analyzed. Results indicated that traditional biochemical analysis and common virulence gene identification might be able to distinguish the strains with different sources. However,PFGE and plasmids analysis could distinguish the sources of strains and to identify those plasmids that commonly carried by the drug-resistant strains. These findings also provided theoretical basis for further study on the nature of resistance and pathogenicity in Proteus mirabilis.
