Clustered regularly interspaced short palindromic repeat associated protein genes cas1 and cas2 in Shigella
10.3760/cma.j.issn.0254-6450.2014.05.025
- VernacularTitle:志贺菌成簇规律间隔短回文重复序列相关蛋白基因cas1和cas2研究
- Author:
Zerun XUE
1
;
Yingfang WANG
;
Guangcai DUAN
;
Pengfei WANG
;
Linlin WANG
;
Xiangjiao GUO
;
Yuanlin XI
Author Information
1. 郑州大学公共卫生学院
- Keywords:
Shigella;
Clustered regularly interspaced short palindromic repeat;
Resistance
- From:
Chinese Journal of Epidemiology
2014;(5):581-584
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the distribution of clustered regularly interspaced short palindromic repeat(CRISPR)associated protein genes cas1 and cas2 in Shigella and to understand the characteristics of CRISPR with relationship between CRISPR and related characteristics on drug resistance. Methods CRISPR associated protein genes cas1 and cas2 in Shigella were detected by PCR,with its products sequenced and compared.Results The CRISPR-associated protein genes cas1 and cas2 were found in all the 196 Shigella isolates which were isolated at different times and locations in China. Consistencies showed through related sequencing appepared as follows:cas2,cas1 (a) and cas1(b)were 96.44%,97.61%and 96.97%,respectively. There were two mutations including 3177129 site(C→G)and 3177126 site(G→C)of cas1(b)gene in 2003135 strain which were not found in the corresponding sites of Z23 and 2008113. Results showed that in terms of both susceptibility and antibiotic-resistance,strain 2003135 was stronger than Z23 and 2008113. Conclusion CRISPR system widely existed in Shigella,with the level of drug resistance in cas1(b) gene mutant strains higher than in wild strains. Cas1(b)gene mutation might be one of the reasons causing the different levels of resistance.
