Investigation on mosquitoes and mosquito-borne viruses in Dehong prefecture,Yunnan province,2007 and 2010
10.3760/cma.j.issn.0254-6450.2014.05.013
- VernacularTitle:云南省德宏州2007年和2010年蚊虫及蚊媒病毒调查
- Author:
Yun FENG
1
;
Hailin ZHANG
;
Shihong FU
;
Weihong YANG
;
Yuzhen ZHANG
;
Piyu WANG
;
Jie YANG
;
Yonghua LIU
;
Chaoliang DONG
;
Shi LI
;
Baosen ZHANG
;
Zhengliu YIN
;
Xingqi DONG
;
Huanyu WANG
;
Guodong LIANG
Author Information
1. 云南省地方病防治所云南省病毒立克次体研究中心
- Keywords:
Mosquito;
Japanese encephalitis virus;
Getah virus;
Culex pipiens pallens densovirus;
Molecular biological characteristics
- From:
Chinese Journal of Epidemiology
2014;(5):528-532
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution patterns of mosquitoes and mosquito-borne viruses in Dehong prefecture,Yunnan province,China. Methods Mosquito samples were collected using the mosquito traps from five counties of Dehong prefecture on July,2007 and 2010. Mosquitoes were cell cultured for viral isolation,and positive isolates were identified using RT-PCR and sequence analysis. Results A total of 43 634 mosquitoes comprised of 29 species representing six genera were collected. Culex tritaeriorhynchus and Anopheles sinensis comprised 78.69% and 14.77% of the total. Six strains of viruses were isolated from the mosquito pools. RT-PCR and phylogenetic analysis revealed three strains from Cx. tritaeriorhynchus,identified as genotypeⅠJapanese encephalitis virus(JEV). One strain was identified from Cx. tritaeriorhynchus,as Getah virus (GETV). Two strains isolated from Cx. tritaeriorhynchus and Anopheles vagus were identified as Culex pipiens pallens Densovirus(CppDNV). Conclusion Cx. tritaeriorhynchus had been the major species of mosquitoes and mainly transmitting vector of mosquito-borne viruses in Dehong prefecture. GenotypeⅠJEV,GETV and CppDNV were the vectors causing transmission of mosquitoe-borne diseases in this area. Data from phylogenetic analysis showed that these newly discovered isolates seemed to have had close relationship with those viruses previously circulating in Yunnan and other provinces of China.
