Isolation of a Specific Antigen from the Triton X-100 Soluble Protein (TSP) Of Mycobacterium tuberculosis.
- Author:
Tae Hyun PAIK
1
;
Hae Sook KWON
;
Sun LEE
;
Ji Sook LEE
;
Eun Kyung JO
;
Hwa Jung KIM
;
Milllina LEE
;
Yung Choon YOO
;
Jung Kyu PARK
Author Information
1. Department of Microbiology, College of Medicine, Konyang University, Nonsan, Chungnam, Korea. thpaik@konyang.ac.kr
- Publication Type:Original Article
- Keywords:
Mycobacterium tuberculosis;
TSP antigen;
40-kDa molecule
- MeSH:
Animals;
Blotting, Western;
Chromatography, Ion Exchange;
Electrophoresis, Gel, Two-Dimensional;
Humans;
Mice;
Mycobacterium tuberculosis*;
Mycobacterium*;
Neptune*;
Octoxynol*;
T-Lymphocytes;
Tuberculosis;
Tuberculosis, Pulmonary;
Vaccines
- From:Journal of Bacteriology and Virology
2004;34(4):273-282
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Tremendous efforts have been made to develop better vaccines and diagnostic markers for the effective control of tuberculosis. Recently, we reported that the Triton X-100 soluble protein (TSP) of Mycobacterium tuberculosis induced strong T-cell proliferation and IFN-gamma production in humans, and also conferred a significant level of protection against tuberculosis in a mouse model. In this study, the TSP was prepared by Triton X-100 extraction of Mycobacterium tuberculosis bacilli, which was followed by Triton X-114 phase partitioning. Western blot analysis using sera of 177 active pulmonary tuberculosis patients and 323 healthy individuals revealed that the TSP contained a immunodominant 40-kDa antigen specifically reacting with some sera from pulmonary tuberculosis patients. The 40-kDa antigen was purified by ion-exchange chromatography, and partially characterized by two-dimensional gel electrophoresis and N-terminal sequencing. Results of this study suggest that 40-kDa molecule of the TSP antigen from the cell suface of Mycobacterium tuberculosis can be used as a serodiagnostic marker as well as a potential vaccine candidate against tuberculosis.
