Tentative study on species-specific oligonucleotide probes for identification of five Bifidobacteria from human being
10.3969/j.issn.1004-8456.2001.03.001
- VernacularTitle:利用种特异性寡核苷酸探针鉴定5种人源双歧杆菌的初步研究
- Author:
Yifan ZHANG
1
;
Lu RAN
;
Xiuzhu DONG
Author Information
1. 国家卫生部食品卫生监督检验所
- From:
Chinese Journal of Food Hygiene
2001;13(3):3-7
- CountryChina
- Language:Chinese
-
Abstract:
For rapidly identifying 5 bifidobacterium species (B.longum,B.breve,B.adolescentis,B.infantis and B.bifidum) often used in functional food,we designed five species-specific oligonucleotide sequences,which were based on the homology analysis of 16s rRNA genes.After being labeled with digoxigenin(DIG) at 3'-end,the sequences were used as probes hybridizing with DNA from these 5 bifidobacteria.These probes showed the desired species specificity,except that the probe for B.infantis had weak cross-reaction with B.breve.The result showed that these 16s rRNA gene probes were specific and effective tools for identifying and detecting Bifidobacteria in food.
