VWF-A1A2A3-mediated expression of P-selectin in platelets under flow shear stress
10.16156/j.1004-7220.2017.02.014
- VernacularTitle:流体剪应力作用下VWF-A1A2A3介导的血小板P-选择素的原位表达
- Author:
Si-Lu LIU
1
;
Xiao-Ling LIU
;
Jian-Hua WU
;
Ying FANG
Author Information
1. 华南理工大学生物科学与工程学院
- Keywords:
Platelet P-selectin;
VWF-A1A2A3;
Flow shear stress (FSS);
Platelet activation
- From:
Journal of Medical Biomechanics
2017;32(2):188-193,204
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of fluid shear stress (FSS) on VWF-A1A2A3-mediated expression of platelet surface P-selectin.Methods Using a parallel plate flow chamber system and mouse anti-human CD62P:FITC as the indicator of P-selectin expression,the alteration of platelet P-selectin expression level with increasing exposure time under different FSS conditions (0,1,2 Pa) specifically mediated by VWF-A1A2A3 was observed and analyzed by fluorescence microscope to obtain the activation characteristics.Results FSS triggered platelet activation and P-selectin expression.The activation ratio of platelet was positively regulated by FSS and their exposure time,reaching the maximum value,9.42％ and 14.59％ under FSS of 1 Pa and 2 Pa,respectively.The level of P-selectin expression exhibited two-phase tendency with the shear stress-exposure time increasing,uplifted at first,then decreased,with the best action time at 7.5 min.The fluorescence peak intensity increased when FSS was enhanced.Conclusions The level of platelet P-selectin expression is co-regulated by VWF-A1A2A3 and FSS,and is closely related to force-signaling exposure time.
