A preliminary study on the expression of caspases in stretch-induced apoptosis in human periodontal ligament cells

VernacularTitle:牵张应变诱导人牙周膜细胞凋亡过程中caspase蛋白酶表达的初步研究
Author: Ya-Qin WU ¹ ; Dan ZHAO ; Jia-Bao ZHUANG ; Chun XU
Author Information

1. 上海交通大学医学院附属第九人民医院口腔修复科
Keywords: Human periodontal ligament cells (HPDLCs); Stretch; Cell apoptosis; Caspases
From: Journal of Medical Biomechanics 2017;32(2):181-187
CountryChina
Language:Chinese
Abstract: Objective To study the expression of caspases in stretch-induced apoptosis in human periodontal ligament cells (HPDLCs).Methods HPDLCs in vitro were subjected to mechanical stretch with 20％ strain for 6 h or 24 h.The apoptotic rates were analyzed by flow cytometry.The protein expression of caspase-3,-5,-7,-8 and -9 was detected by Western blotting,and the activity of caspase-3,-5,-8 and-9 was measured using colorimetric assay.Results Mechanical stretches with 20％ strain for 6 h and 24 h could induce apoptosis in HPDLCs.Compared with non-stretching control group,the protein expression level and activity of caspase-3,as well as the protein expression level of caspase-7 were up-regulated by 24 h-stretch.The protein expression level and activity of caspase-5,-8,-9 were up-regulated after stretches for 6 h and 24 h.Conclusions Mechanical stretch with 20％ strain can induce apoptosis in HPDLCs in vitro,with the activation of caspase-3,-5,-7,-8 and-9.