Silencing Filamin A Inhibits the Invasion and Migration of Breast Cancer Cells by Up-regulating 14-3-3σ
10.1007/s11596-018-1901-6
- Author:
Zhi-Min JI
1
;
Li-Li YANG
;
Juan NI
;
San-Peng XU
;
Cheng YANG
;
Pei DUAN
;
Li-Ping LOU
;
Qiu-Rong RUAN
Author Information
1. Institute of Pathology
- Keywords:
filamin A;
14-3-3σ;
silence;
MAD-MB-231 cells;
invasion;
migration
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2018;38(3):461-466
- CountryChina
- Language:Chinese
-
Abstract:
Filamin A and 14-3-3-σ are closely associated with the development of breast cancer.However,the exact relationship between them is still unknown.The present study aimed to examine the interaction of filamin A with 14-3-3-σ in the invasion and migration of breast cancer.RNA interference technology was employed to silence filamin A in MDA-MB-231 cells.Real-time PCR and Western blotting were used to detect the expression of filamin A and 14-3-3-σ at mRNA and protein levels,respectively.Double immunofluorescence was applied to show their colocalization morphologically.Wound healing assay and Trans-well assay were used to testify the migration and invasion of MDA-MB-231 cells in filamin A-silenced cells.The results showed that silencing filamin A significantly increased the mRNA and protein levels of 14-3-3σ.In addition,double immunofluorescence displayed that filamin A and 14-3-3σ were predominantly colocalized in the cytoplasm of MDA-MB-231 cells.Silencing filamin A led to the enhanced fluorescence of 14-3-3σ.Furthermore,cell functional experiments showed that silencing filamin A inhibited the migration and invasion of MDA-MB-231 cells in vitro.In conclusion,silencing filamin A may inhibit the invasion and migration of breast cancer cells by upregulating 14-3-3σ.
