Monitoring of Biological Responses of Tumor Cells after Irradiation with 99mTc-MIBI —— An In Vitro Study
- Author:
Jinsong XIA
1
;
Hua WU
;
Ming ZHAO
;
Zhiqun XIANYU
Author Information
1. Tongji Hospital Tongji Medical College Huazhong University of Science and Technology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2001;21(2):152-155
- CountryChina
- Language:Chinese
-
Abstract:
To explore the possibility to employ 99mTc-MIBI to monitor biological response of tumor cells after irradiation and to observe the relation between the radiation doses and the uptake levels of 99mTc-MIBI in tumor cells, the cells were irradiated with a single dose of 2 Gy, 10 Gy and 20 Gy respectively. The uptake of 99mTc-MIBI in each dosage group was determined before and 24, 48, 72 h after irradiation respectively. Apoptosis index (AI), plating efficiency (PE) of tumor cells was simultaneously determined. There was a positive correlation between uptake levels of 99mTc-MIBI and AI(r=－0.91, P<0.05). A negative correlation was noted between the uptake levels and PE (r=－0.86, P<0.05). It is suggested that 99mTc-MIBI may be used as a tracer to monitor the change of viability state of tumor cells after being irradiated with different doses.
