Photolithotrophic Cultivation of Laminaria japonica Gametophyte Cells in Stirred Tank Photobioreactors: Studies in Different Pulse Feeding Modes
10.3969/j.issn.1671-8135.2008.01.007
- VernacularTitle:搅拌式光生物反应器培养海带配子体细胞:不同脉冲补料方式的研究
- Author:
Si-Ye CHEN
1
;
Han-Shi QI
Author Information
1. 上海交通大学
- Keywords:
Pulse feeding Laminaria japonica gametophytes Stirred tank photobioreactor Nitrate Phosphate
- From:
China Biotechnology
2008;28(1):36-43
- CountryChina
- Language:Chinese
-
Abstract:
Photoautotrophic gametophyte cells of the brown macroalgae Laminaria japonica were cultivated in 500ml stirred tank photobioreactors under seven pulse feeding modes and one batch mode.It is the first time for the study of effects of the feeding time points and feeding quantity on macroalgal cell growth and nutrient consumption.Results showed that, with inoculum density of 50mg DCW/L, in modified APSW artificial seawater medium at 13℃, light intensity of 60μE/m2.s, light cycle of 16/8h L/D, aeration rate of 50ml/min, and agitation speed of 100r/min, feeding the culture with small nutrient quantity was beneficial for the synchronization between nitrate and phosphate absorption, and further for biomass production.Feeding when ambient nutrient was abundant or depleted was quite weak for large amount of biomass accumulation, which might be due to the slowing nutrient absorption, nutrient storage, or the divergence absorption between nitrate and phosphate.Feeding nutrient frequently with small quantity from mid-exponential growth of macroalgal cells, that is maintaining medium nutrient concentration between 1/3 and 1/2 of its initial concentration, was the most effective way for biomass production, with biomass increased by 12.270 times of for 51 days' cultivation.
