A Novel Biocatalyst and Its Optimized Process for Preparing L-theanine
10.3969/j.issn.1671-8135.2007.10.007
- VernacularTitle:L-茶氨酸新型酶法制备及其催化工艺优化
- Author:
Jia-You LI
1
;
Li-Yun GUO
;
Qing-Cai JIAO
Author Information
1. 嘉兴学院
- Keywords:
L-theanine;
N-Acyl-DL-Theanine;
Cunnighamella echinulata;
L-aminoacylase;
Optical resolution
- From:
China Biotechnology
2007;27(10):34-38
- CountryChina
- Language:Chinese
-
Abstract:
L-theanine (γ-glutamylethylamide) is the main free amino acid component of tea and its favorable physiological effects on mammals have been reported. An enzymatic method for optically pure L-theanine production with a new L-aminoacylases-production fungi Cunnighamella echinulata 9980 was developed. The optimum conditions for enzymatic catalysis were at pH 6.5 with 50 mmol/L N-Acyl-DLtheanine and 40 mmol/L CoCl2. After 12-h incubation at 50℃,22.5 mmol/L L-theanine was obtained, the conversion rate against N-Acyl-L-theanine being 90%. Cunnighamella echinulata and the aminoacylase were applied in preparation of L-theanine.
