Expression of Human Interferon-λ1 and Interferon-ε Gene in WI-38 Cells and Comparison of Their Biological Activity

VernacularTitle:人lambda1和epsilon干扰素基因在WI-38细胞中的表达及其活性比较
Author: Bhushan SARODE ¹ ; Wei-Hua SHENG ; Yu-Feng XIE ; Yao-Dong ZHAO ; Jing-Cheng MIAO ; Ji-Cheng YANG
Author Information

1. 苏州大学
Keywords: Lambdal interferon Epsilon interferon WI-38 cell Antivirus Antiproliferation
From: China Biotechnology 2007;27(3):24-33
CountryChina
Language:Chinese
Abstract: The biological activities i. e. antineoplastic activities and antiviral activity of the two novel kinds of interferons: hIFN-λ1 and hIFN-ε were studied and compared. First the fusion expression vector: pcDNA3.1A-hIFN-λ1-His and pcDNA3.1A-hIFN-ε-His by PCR was constructed, then the two kinds of plasmids were transfected into WI-38 (human embryonic lung cells ) with liposome. And cytopathic effect (CPE) suppression test was used to study and compare the antiviral activities of rhIFNλ1-His and rhIFN-ε-His, meanwhile MTT assay was used to detect their antineoplastic activities. It was found that, antiproliferative activity and MxA protein induction shown by rhIFN-λ1-His is more powerful than of rhIFN-ε -His. The antiviral molecular mechanisms of both hIFN-λ1 and hIFN-ε are related to MxA. The foundation for further study on the bioactivities and mechanism of action of hIFN-λ1 and hIFN-ε was established.