Increasing the Production of IgG in Batch Tissue-culture with IgG-free Ascites
10.3969/j.issn.1671-8135.2005.08.017
- VernacularTitle:用去掉免疫球蛋白IgG的腹水提高体外培养的单克隆抗体产量
- Author:
Ruo-Yu ZHOU
1
;
Jing YE
;
Hong XIE
Author Information
1. Shanghai Institutes for Biological Sciences,The Chinese Academy of Sciences
- Keywords:
Ascites Cell culture IgG production Serum-free media
- From:
China Biotechnology
2005;25(8):87-92
- CountryChina
- Language:Chinese
-
Abstract:
An effective method has been developed for laboratory scale production of IgG. Hybridomas were cultured in serum-free media with 2% IgG-free ascites. Cell density of up to 3.55 × 10 6cells/ml and antibody concentration of 135μ g/ml after purification were abtained, which is four time more than total production of that of IgG concentration in serum-free media. This in vitro method allows great improvement in antibodies production in batch tissue culture. The method reported here is easy to handle and is economical and universally adaptable.
