Correlation between over expression of BUBR1 and chromosomal instability in colorectal carcinoma
10.3969/j.issn.1673-6273.2008.08.001
- VernacularTitle:直结肠癌BUBR1的过度表达与TP53突变及微卫星状态关系提示其过度表达与染色体不稳定表型有关
- Author:
Yan ZHAO
1
;
Tao ZHANG
;
Zhi-Chao ZHENG
;
Jian-Jun ZHANG
;
Yi-Liang ZHAO
;
Yoshihiko MAEHARA
Author Information
1. 辽宁省肿瘤医院
- Keywords:
BUBR1 protein;
colorectal neoplasms;
chromosomal instability;
DNA ploidy;
mierosatellite instability
- From:Progress in Modern Biomedicine
2008;8(8):1401-1405
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Over expression of BUBR1 protein was reported in several human malignancies,however whether BUBR1 plays a role in chromosomal instability phenotype remains in controversy.This study was to explore the roll of BUBR1 protein in CIN phenotype in CRC.Methods:BUBR1 expression was studied immunohistochemieally in a panel of 93 advanced sporadic eolorectal cancers.Microsatellite status was evaluated by high resolution microsatellite analysis assay,TP53 gene mutation by direct sequencing and DNA ploidy by laser scanning cytometery.The relationship between BUBR1 overexpression and TP53 gene mutation,mierosatellite status,and DNA ploidy were studied.Results:BUBR1 overexpression was confirmed in 69% of cases.The overexpression was more frequent in tumor without high frequency microsatellite instability (P＜0.01) and TP53 mutation (P＜0.05).There was no statistic correlation between DNA aneuploidy and BUBR1 overexpression; however,a tendency that aneuploidy tumors had higher percentage of BUBR1 overexpression was shown.BUBR1 overexpression was not statistically related with clinieopathological factors.Conclusion:The linkage between BUBR1 overexpression and molecular factors indicating a CIN background implied that BUBR1 overexpression was indeed related with chromosomal instability in colorectal cancer.
