Effect of berberine on PPARα/δ/γexpression in type 2 diabetic rat kidney
10.3969/j.issn.1673-6273.2008.06.006
- VernacularTitle:小檗碱对2型糖尿病大鼠肾组织PPARα/δ/γ表达的影响
- Author:
Ji-Yin ZHOU
1
;
Shi-Wen ZHOU
;
Jian-Lin TANG
;
Yi YING
;
Ying XU
;
Le ZHANG
;
Dan-Dan LI
Author Information
1. 第三军医大学附属新桥医院
- Keywords:
Berberine;
PPARα/δ/γ;
Diabetes mellitus;
Immunohistocbemistry
- From:
Progress in Modern Biomedicine
2008;8(6):1020-1023,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of peroxisome proliferator-activated receptors (PPARs) α/δ/γ protein and the effect of berberine on them in diabetic rat kidney.Methods Type 2 diabetic rats were induced by injection (ip) with streptozotocin 35 mg·kg1 and feeding with a high-carbohydrate/high-fat diet for 16 weeks.From week 17 to 32, diabetic rats were given low, middle, high-dose berberine 75, 150, 300 mg×kg-1, fenofibrate 100 mg×kg1 and rosiglitazone 4 mg×kg-1, respectively.The expression of PPARα/δ/γ protein in kidney were detected by immunohistochemistry.Results The expression of PPARα and PPARδ protein in the kidney of diabetic rat, which were significantly higher than that of the control one (P＜0.01), while PPARγ expression was obviously lower than that of the control one (P＜0.01).The expression of PPARα and PPARδ in in diabetic kidney were increased notably after middle-dose, high-dose berberine and fenofibrate treatment (P＜0.01);middle-dose, high-dose berberine and rosiglitazone treatment significantly reduced PPARγ expression (P＜0.01).Conclusion Berberine can restore the disturbed PPARα/δ/γ protein expression in diabetic rat kidney.
