Optimal Supply of Precursors for CoQ10 Production by Rhodopseudomonas Palustris
10.3969/j.issn.1673-6273.2008.05.014
- VernacularTitle:对沼泽红假单胞菌生产CoQ10添加前体物的优化
- Author:
Shi-Yun JIANG
1
;
Long-Jiang YU
;
Xin XIONG
;
Xiao-Lin SHEN
;
Yan JIAN
Author Information
1. 华中科技大学
- Keywords:
CoQ10;
Response surface methodology;
Rhodopseudomonas palustris;
Methionine;
4-Hydroxybenzoic acid;
Solanesol
- From:
Progress in Modern Biomedicine
2008;8(5):845-850
- CountryChina
- Language:Chinese
-
Abstract:
CoQ10 has been used not only as a medicine but also as food supplements because of its various physiological and biochemical activities. A full-factorial central composite design and response surface methodology were used for optimizing three precursors Solanesol, 4-hydroxybenzoic acid and methionine to maximize the production of CoQ10 by Rhodopseudomonas palustris J001. The optimization of the model predicted a maximum response 40.6 [(mg CoQ10)(g dried biomass)-1] CoQ10 production with 124.8 mg l-1 Solanesol, 267.7 mg l-14-hydroxybenzoic acid and 130.2 mg l-1 methionine, respectively. A new combination was prepared according to the result. The observed response was 40.5 ± 0.2 [(mg CoQ10)(g dried biomass)-1] and was 109.8%higher than in the control with no addition of the three precursors.
