Effects of Stainless Steel and Titanium Oxide in Stimulation of Endothelial Cells Releasing NO and MCP-1

VernacularTitle:不锈钢和氧化钛薄膜刺激内皮细胞释放NO和MCP-1的研究
Author: Gui-Cai LI ¹ ; An-Sha ZHAO ; Ping YANG ; Nan HUANG
Author Information

1. 西南交通大学
Keywords: Titanium oxide; Stainless steel; Blood vessel stents; MCP-1; N0
From: Progress in Modern Biomedicine 2008;8(5):836-838
CountryChina
Language:Chinese
Abstract: To investigate the influence of stainless steel(SS)and titanium oxide(Ti-O)thin films on the cytokine released by en-dothelial cells.Ti-O films were prepared by unbalanced magnetron sputtering deposition system(UBMS).Human umbilical vein en-dothelial cells(HUVECS)were isolated and seeded on SS and Ti-O materials.Nitride oxide (NO)and monocytc chemotactic protein one(MCP-1)concentrations were determined in the supematants with enzyme linked immunosorbent assay(ELISA)at 1,3 and 5 days after culture,and the morphology of the cells was observed using scanning electron microscopy(SEM).The results showed that the similar amount of HUVECs was presented in SS and Ti-O culture.But HUVEC had better morphology on Ti-O film than SS.The NO and MCP.1 test also showed that HUVECs on Ti-O film had a better bio-function than SS.It is concluded that Ti-O film as a biomaterial may have a better endothlieal compatibility,which can be used to enhance the endothelialization.