Effects of Glycosaminoglycan From Scallop Skirt on The Expression of Vascular Endothelial Growth Factor During The U937 Foam Cell Formation
10.3969/j.issn.1673-6273.2007.06.015
- VernacularTitle:扇贝裙边糖氨聚糖对泡沫细胞形成过程中血管内皮生长因子表达的影响
- Author:
Fu-Sheng SUN
1
;
Sai LIU
;
Chuan-Xia JU
;
Nan YU
Author Information
1. 山东省青岛市立医院
- Keywords:
Glycosaminoglycan from Scallop Skirt;
Foam cell;
Vascular endothelial growth factor;
Atherosclerosis
- From:Progress in Modern Biomedicine
2007;7(6):846-848
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of glycosaminoglycan from scallop skirt (SS-GAG) on the expression of vascular endothelial growth factor (VEGF) and the mechanism of anti-atherosclerosis action of SS-GAG. Methods: U937 cells were incubated with 80mg/L oxidized low density lipoprotein (ox-LDL) for 48h to establish a macrophage-derived foam cell model. In addition, U937 cells were divided into 6 groups: ①control group; ②ox-LDL group; ③ox-LDL+200mg/L SS-GAG group; ④ox-LDL+400 mg/L SS-GAG group; ⑤ox-LDL+800 mg/L SS-GAG group; ⑥ox-LDL +Heparin 100 mg/L group.After 48h's incubation, the concentration of VEGF in the medium was determined by ELISA. Results: The expression of VEGF in U937 foam cells was obviously higher than that of the control group. After treatment with heparin (100 mg/L) and SS-GAG of different concentrations (200mg/L, 400 mg/L, 800 mg/L), the expression of VEGF decreased obviously, especially in the ox-LDL+800 mg/L SS-GAG group (P＜0.01). Conclusion: The antiatherogenic effect of SS-GAG is probably due to its ability to inhibit VEGF expression.
