Reconstruction of Tissue Engineering Skin by Epidermal Cells and Fibroblasts Combined with Modified Polymer of Lactic Acid

VernacularTitle:皮肤细胞复合改性聚乳酸构建组织工程皮肤
Author: Ying FENG ¹ ; Zong-Liang WANG ; Tie-Ying SHI ; Yi SHI ; Yu-Lai ZHOU ; Wei-Qun YAN
Author Information

1. 吉林大学
Keywords: Polymer of lactic acid; Dialdehyde starch; Tissue engineering skin
From:Progress in Modern Biomedicine 2007;7(5):711-713
CountryChina
Language:Chinese
Abstract: Objective: To investigate the applied feasibility of scaffold with modified PLA (Polymer of lactic acid) in tissue engineering. Methods:First, we adopted salting-in method to prepare porous foam scaffold. Then, we reconstructed tissue engineering skin by epidermal cells and fibroblasts combined with modified PLA. On the 14th day of cell culturing in vitro, we was a control. Results:The arfificial skin is composed of epidermis and dermis and similar to natural skin in appearance. The skin consists of fibroblasts and keratinocytes, which are in various proliferation and differentiation stages. Fibroblasts and keratinocytes distribute on the surface of polymer of lactic acid (PLA) and the number of fibroblast and keratinocyte increase. Conclusion:Dialdehyde starches (DAS) not only improve the function of PLA but also have good effects on cells. Moreover, it does not affect the growth and the metabolism of the cells. So it is feasible to use modified scaffold to construct tissue engineering skin.