Role of BALF from dogs with acute severe smoke inhalation injury in ind ucing lung injury of normal rats
10.3321/j.issn:1000-5404.2001.05.022
- VernacularTitle:犬烟雾吸入伤早期肺洗出液对大鼠肺损伤作用的研究
- Author:
Fa-Chuan NIE
1
;
Zong-Cheng YANG
;
Zhi-Yuan LIU
;
Qi-Zhi LUO
;
E-sheng Yu HUANG
Author Information
1. 第三军医大学附属西南医院
- From:Journal of Third Military Medical University
2001;23(5):559-561
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the existence, intensit y and persisting time of biologic activity of bronchoalveolar lavage fluid (BALF ) collected from dogs with acute severe smoke inhalation injury in early post-i njury stage. Methods BALF was collected 1 h after the est ablishment of acute severe sawdust smoke inhalation injury in 5 dogs, and the fl uid was perfused into the lungs of Wistar rats in the amount of 5 ml/kg (gro up C). Normal saline (group A) and BALF from normal dog (group B) were perfused into the lungs of rats and served as control. The respiratory rate, PaO2, lung water content and the expanding stability of lungs in all rats were determ ined at the time points of 4,12 and 24 h after the purfusion. Results Compared with the rats in group A and B, the rats in group C had higher mortality, wider range in RR, higher lung water content, PaO2 decreased obviou sly and lower lung expanding stability. The rats in control groups showed sl i ght mechanic obstruction in their airways in the course of experiment. Meanwhile ，the rats in group C showed higher oxidative activities and lower total anti-o xidative activities in lung tissues. Conclusion It is certai n that the BALF collected from dogs with acute severe smoke inhalation injury in early post-injury stage could induce obvious injury in lung structure of norma l rats, showing certain mechanic obstruction in small airways. The injuring act ivity of the BALF can be alleviated ultimately 24 h after the perfusion of the B ALF.
