Antiproliferative effect of soybean isoflavone on Bcap-37 cells and its relation with transforming growth factor β
10.3321/j.issn:1000-5404.2001.04.021
- VernacularTitle:大豆异黄酮抑制Bcap-37细胞增殖与TGF-β关系的研究
- Author:
Zhen-Zhou YANG
1
;
Man-Tian MI
;
Jun-Dong ZHU
;
Qian-Yong ZHANG
;
Ji-Guang XIONG
Author Information
1. Third Military Medical University
- From:Journal of Third Military Medical University
2001;23(4):434-436
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of TGF-β and TGF-β receptor in human breast cancer cell Bcap-37 inhibited by soybean isoflavones. Methods mRNA and protein of TGF-β1、TGF-βRⅠ in Bcap-37 cells were examined with reverse transcription ploymerase chain reaction(RT-PCR) and immunohistochemistry after cells were treated with daidein or genistein for 1-4 d.The expression of TGF-β1 and TGF-β2 was determined with TGF-β resistance test. Results The TGF-β1, TGF-β2 and TGF-β recepor increased in Bcap-37 cells at a concentration of 3×10-5 mol/L of genistein. No changes was found when treated with daidzein. Conclusion Genistein may inhibit the proliferation of Bcap-37 cells and accompany with increasing expression of TGF-β1, TGF-β2 and TGF-β receptor.
