Role of β-endorphin in conA-induced spleen cell proliferation in rats with traumatic hemorrhagic shock
10.3321/j.issn:1000-5404.2001.04.011
- VernacularTitle:β-内啡肽在大鼠创伤失血性休克脾细胞增殖功能的作用研究
- Author:
Ai-Qing WEN
1
;
Liang-Ming LIU
;
De-Yao HU
Author Information
1. Research Institute of Field Surgrey Daping Hospital Third Military Medical University
- From:Journal of Third Military Medical University
2001;23(4):404-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of β-endorphin (β-EP) in conA-induced spleen cell proliferation after traumatic hemorrhagic shock. Methods ①Wistar rats with traumatic hemorrhagic shock were used and killed 0, 1, 3, 6,12 and 24 h after traumatic hemorrhagic shock. Plasma specimens were collected and β-EP levels in plasma were detected. Rats with sham-operation served as the control. ②Spleen cells isolated from normal rats were cultured in shock plasma (group Ⅰ), inactivated shock plasma (group Ⅱ) and shock plasma+β-EP antiserum (group Ⅲ) respectively. Con A-induced spleen cell proliferation was observed. Results ①The plasma β-EP level was elevated significantly immediately after shock, and reached the peak 1 h later, then showed a deceasing tendency and restored to the level as before shock at 24 h. ②Shock plasma remarkedly suppressed spleen cell response to the mitogen conA (P<0.01) compared with control; ConA-induced spleen cell proliferative function in group Ⅱ was significantly increased than that in group Ⅰ (P<0.01), so did in group Ⅲ, which still lower than in control. Conclusion The significantly elevated β-EP in the plasma after hemorrhagic shock might play an important role in inhibiting the proliferation of spleen cells.
