Effect of cardiopulmonary bypass serum on pulmonary surfacant-associated protein A and the preventive effect of pentoxifylline
10.3321/j.issn:1000-5404.2001.03.028
- VernacularTitle:体外循环血清对肺表面活性物质相关蛋白A的影响及己酮可可碱的作用
- Author:
Jia HAO
1
;
Ying-Bin XIAO
;
Hong GUO
;
Qian-Jin ZONG
;
Xue-Feng WANG
;
Lin CHEN
Author Information
1. Xinqiao Hospital Third Military Medical University
- From:Journal of Third Military Medical University
2001;23(3):340-342
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the injurious effect of the serum drawn from patient under cardiopulmonary bypass (CPB) on pulmonary surfacant-associated protein A(SP-A) and the preventive effect of pentoxifylline (PTX) on the protein. Methods The cultured rat alveolar epithelial cell type Ⅱ(AT-Ⅱ) were incubated with CPB serum to observe the change of the cell morphology, and the expressions of SP-A and SP-A mRNA. Results Traumatic changes and the decrease of SP-A and SP-A mRNA expressions were found in AT-Ⅱ cells cultured with CBP serum. PTX exerted protective effect on the cells. Conclusion The serum after CPB can directly injure rat AT-Ⅱ cells in vitro, and inhibit the SP-A expression at transcription and translation levels, which probably is an important reason for the quantitative and qualitative abnormality of pulmonary surfactant after operation. PTX may alleviate the inhibitory effect of CPB serum on SP-A.
