Experimental study of astrocyte reaction to injury in vitro
10.3321/j.issn:1000-5404.2001.03.020
- VernacularTitle:星形胶质细胞对损伤反应的体外实验研究
- Author:
Qi-Lin HUANG
1
;
Wen-Qing CAI
;
Ke-Cheng ZHANG
Author Information
1. Xinqiao Hospital Third Military Medical University
- From:Journal of Third Military Medical University
2001;23(3):315-317
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the occurence, development and regulation of reactive gliosis with astrocyte (Ast) in vitro. Methods Ast was isolated and cultured in vitro and its model of reactive gliosis was established by scratching the cultured astrocytes. The reactivity and rules of Ast to injury was studied by morphological changes, RT-PCR, immunocytochemistry, in situ hybridization and imaging analysis. Results After scratching, the astrocytes showed typical features of reactive gliosis, with the hypertrophic cell body, thickened and lengtheded processes, and enhanced glial fibrillary acidic protein (GFAP) staining. In situ hybridization and RT-PCR analysis confirmed that the expression of GFAP mRNA was markedly increased. These changes occurred 1 d after scratching and reached the peak 5 to 7 d after injuring. Conclusion A model of reactive astrogliosis was successfully established in vitro which showed an active reaction to injury. The characteristics of reactive gliosis parallel that seen in vivo.
