Expression of some oncogenes and point mutation of c-Ha-ras1 during hepatocarcinogenesis in rats
10.3321/j.issn:1000-5404.2001.03.018
- VernacularTitle:大鼠肝癌变过程中几种癌基因的原位表达及c-Ha-ras1点突变的研究
- Author:
Xin-Li ZHANG
1
;
Jing-Quan SHI
;
Xiu-Wu BIAN
Author Information
1. Research Institute of Pathology Southwest Hospital Third Military Medical University
- From:Journal of Third Military Medical University
2001;23(3):308-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expressions of oncogenes c-Ha-ras, c-ki-ras, pan-ras and c-myc and point mutation of c-Ha-ras1 during hepatocarcinogenesis in rats. Methods Immunohistochemistry, in situ hybridization and microdissection of tissue (MDT)-PCR-SSCP were used to detect the oncogene expressions and point mutation of c-Ha-ras1 in both Solt-Farber model and DEN-induced liver cancer model. Results The overexpression of c-Ha-ras was closely associated with the formation and proliferation of the precancerous basophilic hepatocyte foci, while that of c-myc with the growth of the oval cell foci. The abnormalities of IGF-Ⅱ played an important role in the evolution of precancerous foci/nodules towards hepatocellular carcinoma (HCC). The overexpression of fms was only associated with HCC of some rats. Conclusion Hepatocarcinogenesis in rats was related with the overexpression of c-Ha-ras, c-myc, IGF-Ⅱand fms and the point mutation of c-Ha-ras1, and overexpression of these oncogenes was associated with morphological evolution.
