Effects of nordihydroguaiaretic acid treatment combined with Methotrexate or Vincristine on a human malignant glioma cell line
10.3321/j.issn:1000-5404.2001.03.011
- VernacularTitle:去甲二氢愈创木酸与氨甲喋呤、长春新碱配伍对人恶性胶质瘤细胞株的作用
- Author:
Jian CAO
1
;
Fu WU
Author Information
1. Xinqiao Hospital Third Military Medical University
- From:Journal of Third Military Medical University
2001;23(3):284-286
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of nordihydroguaiaretic acid treatment combined with Methotrexate or Vincristine on a human malignant glioma cell line. Methods The effect of drugs on the cells was measured by MTT, and the protein expression of cyclin D1 gene in the cells was measured by immunohistochemistry assay. Results ①The anti-tumor effects of drugs on the cells were increased with the increasing of concentrations, and had a combined function between NDGA and MTX or VCR. ②There was no difference between the effect of treating NDGA with MTX or VCR given at the same time and MTX or VCR given after 24 h of NDGA treatment, but the effects were apparently different when NDGA was administrated after MTX or VCR treatment for 24 h. ③The protein expression of cyclin D1 gene in the cells was apparently reduced after NDGA treatment. Conclusion There is a cooperative function between NDGA and MTX or VCR on the cells, and the function is probably related to the NDGA-induced down-regulation of protein expression of cyclin D1 gene in the cells.
