Effect of nordihydroguaiaretic acid on the morphological changes of endothelial cells in vitro
10.3321/j.issn:1000-5404.2001.03.010
- VernacularTitle:去甲二氢愈创木酸对内皮细胞影响的形态学观察
- Author:
Hui-Qin SUN
1
;
Zhao-Zheng CHEN
;
Yi-Sheng CHEN
;
Jing-Quan SHI
;
Xiu-Wu BIAN
;
Rong XIN
Author Information
1. Research Institute of Pathology Southwest Hospital Third Military Medical University
- From:Journal of Third Military Medical University
2001;23(3):280-283
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate morphological changes of endothelial cells after nordihydroguaiaretic acid (NDGA) treatment in vitro. Methods The morphological changes of human umbilical vein endothelial cells (HUVEC) cell line ECV-304 and the cell apoptosis rate in sub-G0 phase were observed with invert, light and electron microscope and flow cytometry after NDGA treatment at different concentrations or with PBS (0.01 mol/L) as control. Results ①After the treatment of NDGA at 50～200 μmol/L for 1～3 d or up to 8 d at 100 μmol/L, ECV-304 cells tended to elongate into a shuttle-like sparse appearance and those in mitosis were decreased, indicating the suppression of cell proliferation. All these alteration was in a time-and dose-dependent manner. ②NDGA-treated ECV-304 cells displayed morphological features of apoptosis, especially at the 48th h after the treatment. With flow cytometry, the cells in sub-G0 phase were significantly increased, and reached its peak at hour 12 (20.42%) after NDGA treatment. In addition, the degeneration and necrosis of ECV-304 cells were related to the concentrations of NDGA. Conclusion NDGA can inhibit the proliferation and growth of endothhelial cells, and induce apopotosis, which might also inhibit angiogenesis.
