Effect of nordihydroguaiaretic acid on expressions of VEGF and its receptor KDR in vitro
10.3321/j.issn:1000-5404.2001.03.007
- VernacularTitle:去甲二氢愈创木酸对VEGF及其受体KDR基因表达影响的研究
- Author:
Hui-Qin SUN
1
;
Yi-Sheng CHEN
;
Jing-Quan SHI
;
Xiu-Wu BIAN
;
Zhong-Min ZOU
;
De-Yu GUO
;
Rong XIN
Author Information
1. 第三军医大学附属西南医院
- From:Journal of Third Military Medical University
2001;23(3):268-271
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of nordihydroguaiaretic acid (NDGA) on the expressions of vascular endothelial growth factor (VEGF) and its receptor, kinase-inserted domain containing receptor(KDR) and the possible mechanism. Methods The expression of VEGF in human malignant glioma cell line SHG-44 and that of KDR in human umbilical vein endothelial cell (HUVEC) line ECV-304 were observed 1～3 d after NDGA treatment with immunohistochemistry, in situ hybridization and image analysis. Results The expression of VEGF was declined at protein or mRNA levels in SHG-44 cells after treated with 100 μmol/L NDGA for 1 to 3 d. The expression of KDR in endothelial cells with 100 μmol/L NDGA treatment for 1 to 3 d was decreased too, in a more obvious way compared with the decline of VEGF expression in SHG-44 cells. Conclusion The results suggest that NDGA inhibits the expression of VEGF in glioma cells as well as that of VEGF receptor KDR in endothelial cells, which may be the important molecular mechanism of anti-angiogenesis of NDGA.
