Effect of vascular endothelial growth factor on apoptosis of endothelial cells induced by hypoxia
10.3321/j.issn:1000-5404.2001.02.025
- VernacularTitle:血管内皮生长因子对缺氧诱导内皮细胞凋亡的影响
- Author:
Jun JIN
1
;
Shan-Jun ZHU
;
Lan HUANG
;
Chang-Qing XIANG
;
Hong LI
Author Information
1. Xinqiao Hospital Third Military Medical University
- From:Journal of Third Military Medical University
2001;23(2):196-198
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of hypoxia on the apoptosis of cultured human umbilical vein endothelial cells（HUVECs） and the role of vascular endothelial growth factor（VEGF） in inhibition of apoptosis． Methods ①Culture and identification of HUVECs．②Establishment of hypoxic model（0，12，24，48 h）in HUVECs．③Incubation of HUVECs with VEGF(0 ng, 100 ng) under hypoxic condition for 24 h. ④Detection of apoptosis of HUVECs with TUNEL method． Results The percentages of apoptosis were different under different hypoxic conditions． The longer hypoxic time was，the higher apoptosis percentage was．VEGF reduced the apoptosis of HUVECs induced by hepoxia． Conclusion Over-apoptosis EVCs in one of the important factors for the impairment of endothelial function. HEGF inhibits the apoptosis of HVCs and having a pretive function on them.
