Concentration of Ca2+, contents of cAMP, CaM and Ca2+/CaM-PK II in pheochro-mocytoma PC12 cells after combined soman and hypoxia injury
10.3321/j.issn:1000-5404.2001.02.017
- VernacularTitle:缺氧复合梭曼中毒PC12细胞中[Ca2+]、cAMP、CaM和Ca2+/CaM-PK Ⅱ的变化
- Author:
Ji-Qing ZHAO
1
;
Qiang WU
;
Shi-Li WANG
;
Xiang-De WEI
;
Zhao-Jun DONG
;
Yun-Peng LI
;
Yong LIU
Author Information
1. Third Military Medical University
- From:Journal of Third Military Medical University
2001;23(2):169-171
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of the concentration of Ca2+, contents of cAMP, CaM and activity of Ca2+/CaM-PK II in pheochromocytoma PC12 cells after combined soman and hypoxia injury. Methods The changes of [Ca2+], and activity of CaM, cAMP and Ca2+/CaM-PK II in PC12 cells were studied after combined soman and hypoxia injury with radioimmunoassay. Results The changes of [Ca2+], the contents of CaM, cAMP were significantly higher in hypoxic and soman intoxicated group than in soman intoxicated group and control group under hypoxia; but the activity of Ca2+/CaM-PK Ⅱ were significantly decreased. Conclusion [Ca2+], CaM, cAMP and Ca2+/CaM-PK Ⅱ exert important role in the damage of PC12 after combined soman and hypoxia injury.