Study on epithelial-mesenchymal transition in the early stage of mice renal interstitial fibrosis
10.3321/j.issn:0258-879X.2005.04.003
- VernacularTitle:小鼠肾间质纤维化早期上皮细胞-间充质细胞转型研究
- Author:
Jun XIONG
1
;
Kai-Meng HU
;
Kai-Hong JI
;
Shu-Ping TANG
;
Yin WANG
;
Hou-Qi LIU
Author Information
1. 第二军医大学
- Keywords:
renal interstitial fibrosis;
myofibroblast;
renal basement membrane;
matrix metalloproteinase
- From:
Academic Journal of Second Military Medical University
2005;26(4):355-360
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe morphological changes of epithelial-mesenchymal transition in the early stage of mice renal interstitial fibrosis. Methods: Renal interstitial fibrosis was induced by unilateral ureteral obstruction(UUO) in mice. Histological and immunohistochemical methods were used to analyze pathological changes and α-SMA expression in renal tissue.Argentum hexamethylenamine staining and transmission electron microscopy were used to observe changes of the renal tubule basement membrane. Gelatin zymographic analysis was used to observe the expression of MMP2 and MMP9 in renal tissue.Results:The mice suffered from renal interstitial fibrosis were identified by histological analysis and α-SMA positive cells in renal tissue. Argentum hexamethylenamine staining and transmission electron-microscopy showed that the renal tubule basement membrane disrupted locally and renal tubule epithelial cells invaded into the renal interstitium in the early stage of renal interstitial fibrosis. Gelatin zymographic analysis showed that the expression of MMP2 and MMP9 was increased transitorily in the early stage of renal interstitial fibrosis. Conclusion: Renal tubule basement membrane disruption, renal tubule epithelial cells invasion into the renal interstitium, and the expression of MMP2 and MMP9 are involved in the development of renal interstitial fibrosis.
