Effect of intracellular-free calcium changes on the process of pancreatic cancer cell line apoptosis induced by As2O3
10.3321/j.issn:0258-879X.2001.05.007
- VernacularTitle:细胞内Ca2+变化在砷剂诱导胰腺癌细胞株凋亡中的作用
- Author:
Xing-Rong ZHANG
1
;
Hong-Pei CAI
;
Zhi-Hua DENG
;
Jian-Wei SHEN
Author Information
1. Changzheng Hospital Second Military Medical University
- From:
Academic Journal of Second Military Medical University
2001;22(5):422-424
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of intracellular-free calcium and the expression of Fas and Fas L on the process of pancreatic carcinoma cell apoptosis. Methods: Apoptosis induced by 2 μmol/L arsenic trioxide in pancreatic cancer cell lines SW-1990 was investigated.Concentration of intracellular-free calcium （［Ca2+］i） was determined by Fura-2a fluorescein load technique. Fas and FasL were determined by flow cytometry. Results: Pancreatic cancer cells treated with 2 μmol/L arsenic trioxide presented apoptotic features: intact cell membrane, chromatin condensation, nucleic fragmentation and apoptotic body formation; agarose electrophoresis showed marked ladder; flow cytometery analysis showed a sub-G1 cell peak. In the process of pancreatic carcinoma cell apoptosis Fas and FasL and the ［Ca2+］i were significantly higher than that in the control. Conclusion: The pancreatic cancer cell apoptosis induced by arsenic trioxide is related to Fas and FasL expression by the cancer cells and the ［Ca2+］i increase in the cancer cells.
