Effects of negative PTFE electrets on the apoptosis of fibroblast cells

VernacularTitle:负极性聚四氟乙烯驻极体对成纤维细胞凋亡的影响
Author: Jian JIANG ¹ ; Cheng-Rong SONG ; Li-Li CUI ; Xiao-Ping WANG ; Ying FANG ; Li LI ; XI-Ting YE
Author Information

1. Second Military Medical University
From: Academic Journal of Second Military Medical University 2001;22(5):409-411
CountryChina
Language:Chinese
Abstract: Objective: To study the influence of negative electrets on apoptosis of fibroblast cells and to probe its mechanism. Methods: Fibroblast cell were treated with -300, -500 and -1 000V PTFE electrets for 24, 48 and 72 h, respectively, and the influence of negative electrets on cell apoptosis was studied by means of flow cytometry and transmission electron microscope. Results: Compared with control group, apoptosis cells increased from 0.5% to 10% (some even to 15%) after 24，48 and 72 h action of -300, -500 and -1 000 V electrets. After action of -500 V PTFE electrets for 48-72 h, fibroblast cells showed characteristic morphological features of apoptosis. These features included chromatin aggregation, nuclear and cytoplasmic condensation and partition of cytoplasm and nucleus into membrane bound-vesicles (apoptotic bodies). The effect of negative electrets on apoptosis was in proportion to the time and electric field intensity. Conclusion: Negative electrets can enhance apoptosis of fibroblast cells.