Detecting telomerase activity of gastrointestinal tract cancerous cell lines by TRAP-ELISA
- VernacularTitle:TRAP-ELISA检测消化道肿瘤细胞株端粒酶活性
- Author:
Hong-Pei CAI
1
;
Zhi-Hua DENG
;
Xing-Rong ZHANG
;
Yong GAO
;
Jian-Wei SHEN
Author Information
1. Changzheng Hospital Second Military Medical University
- From:
Academic Journal of Second Military Medical University
2001;22(4):378-380
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To select the telomerase positive cancer cell lines of gastrointestinal tract and to provide a convinced methodology for future telomerase study. Methods: Fifteen cancer cell lines (carcinoma of stomach 4, of liver 6, of pancreas 2, of colon 3) were cultured and telomerase activity were detected by TRAP-ELISA. The normal hepatic cells were taken as control. Results: Thirteen cell lines were telomerase positive in the 15 lines(86.7%). Conclusion: Most of gastrointestinal tract cancer lines express telomerase, indicating the detection of telomerase activity has clinical potential.
