Preparation and test of type Ⅰ collagen-glycosaminoglycan (GAG) template
10.3321/j.issn:0258-879X.2001.04.014
- VernacularTitle:Ⅰ型胶原-糖胺聚糖模板的制备与检测
- Author:
Qing-Lei XU
1
;
Hai-Shan WU
;
Wei-Jiang ZHOU
;
Ding－lin ZHAO
Author Information
1. Changzheng Hospital Second Military Medical University
- From:
Academic Journal of Second Military Medical University
2001;22(4):337-339
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To synthesize the collagen-GAG template and to evaluate its feasibility to be used as the MSCs vehicle for meniscal tissue engineering. Methods: The collagen-GAG template was synthesized from rat tail type Ⅰ collagen and GAG using Yannas method. Then the post-stimulated MSCs by bFGF and TGF-β1 were added in. The MSCs-enriched collagen sponges were cultured in vitro， two weeks later the histological and ultrastructure detection was performed. Results: The histological and ultrastructure of the collagen-GAG template remained intact after 2 weeks' culture, and the MSCs in it remained viable. Conclusion: The collagen-GAG template synthesized in this experiment is suitable for the meniscal tissue engineering reconstruction as the vehicle for MSCs seed cells.
