Biological properties of the mouse embryonic stem cell line expressing green fluorescent protein
10.3321/j.issn:0258-879X.2001.04.009
- VernacularTitle:表达绿色荧光蛋白的小鼠胚胎干细胞系的生物学特性
- Author:
Hua YANG
1
;
Ji-Liang FU
Author Information
1. Second Military Medical University
- From:
Academic Journal of Second Military Medical University
2001;22(4):322-324
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the characteristics of mouse embryonic stem (ES) cell line R1 expressing green fluorescent protein (GFP) efficiently and stably. Methods: Four kinds of biological characteristics: growth curve, express level of alkaline phosphatase, karyotype and pluripotentiality of the subclones of R1 ES cell expressing GFP were observed and analysed. Results: No obvious differences between R1 ES cells and the 4 ES cell clones expressing GFP［ESG（+）］ on the proliferation speed, differentiation state and the ratio of normal karyotype were observed. Teratocarcinoma concluded 3 germinal layers could form after inoculating ESG(+) cells to nude mice. Conclusion: The expression of GFP may not make any detectable effect on the proliferation speed, differentiation state and the pluripotentiality of R1 ES cells. This research work ensures efficient utilization of GFP as a reporter gene tracing ES cell in vivo.
