Effects of probucol on the NF-κB-like activity of aortic smooth muscle cell in vitro
10.3321/j.issn:0258-879X.2001.02.015
- VernacularTitle:普罗布考对体外培养的主动脉平滑肌细胞NF-κB活性的影响
- Author:
Jiang-Tao GU
1
;
Zong-Gui WU
;
Qian SHEN
;
Yu-Lian XU
Author Information
1. Changzheng Hospital Second Military Medical University
- From:
Academic Journal of Second Military Medical University
2001;22(2):151-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective：To observe the inhibitory effects of pr obucol on nuclear factor-κB （NF-κB）-like activity of vascular smooth mus cle cells(VSMCs). Methods:The effects of probucol and H2O2 on NF-κB-like activity of VSMCs were investigated by electrophoretic mobility shift assay(EMSA). Results:NF-κB activation of VSMCs could be induced by H2O2 or new born calf serum(NCS) for 72 h, the gray-measure of the protein bindings measured through computer scanning was 63.9 and 46.6 respe c tively. NF-κB activity of VSMCs stimulated by H2O2 or NCS might be prevent ed by 100 μmol/L probucol, the inhibitory rate was 37.1%, 14.8% respectively. Conclusion:probucol can inhibit NF-κB activity of VSMCs stim ulated by NCS or H2O2. These effects of probucol might help to re duce development of atheroclerosis and restenosis after percutaneous transluminal coronary angioplasty.
