Effects of oxLDL and simvastatin on PKC activity and level of cytosolic free Ca 2+ in cultured human umbilical vein endothelial cells
10.3321/j.issn:0258-879X.2001.02.012
- VernacularTitle:辛伐他汀及oxLDL对人脐静脉内皮细胞蛋白激酶C活性和胞质内游离钙水平的影响
- Author:
Jin-Chuan YAN
1
;
Zong-Gui WU
;
Ling-Zhen ZHANG
;
Li LI
;
Jie FAN
;
Ling LING
;
Wen-Yu HAN
;
Suo-Long ZHANG
Author Information
1. Changzheng Hospital Second Military Medical University
- From:
Academic Journal of Second Military Medical University
2001;22(2):140-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of oxLDL and HMG-CoA reductase inhibitor simvastatin on PKC activity, and level of cytosol ic free Ca 2+ in cultured human umbilical vein endothelial cells. Methods: Th e activity of PKC was determined by its ability to transfer phosphate from 32P-ATP to lysine-rich histone and level of cytosolic free calcium［Ca2+ ］i was measured by flow cytometric analysis loading with the Ca2+ dye F luo-3/Am. Results: oxLDL increased PKC total activity in a dose-de pendent manner and peaked after 12 min, then decreased slowly and maintained for at least 30 min, while oxLDL induced biphasic ［Ca2+］i responses includ ing the rapid initial transient phase and the sustained phase. Removal of extrac ellular Ca2+ did not inhibit the rapid transient phase, but abolished the sustained phase. When simvastatin was added, the activity of PKC wasmarkedly dec reased with no impairment to the initial peak response, but significantly reduce d the sustained phase. Conclusion: oxLDL can induced dynamic changes of signal transduction of PKC and level of cytosolic free Ca2+ in HUVEC, these 2 events are closely linked. The change of rapid initial transient phase i s the result of mobilization of Ca2+ from intracellular pool and the chang e of sustained phase is from the influx of extracellular Ca2+. The inhibit ion of PKC activity induced by simvastatin may contribute to the changes of ［Ca 2+］i.
