Effects of cytokines on the expression of CD40 and CD40 ligand in cultured human monocytes/macrophages
10.3321/j.issn:0258-879X.2001.02.008
- VernacularTitle:细胞因子对人单核/巨噬细胞表达CD40和CD40L的影响
- Author:
Jin-Chuan YAN
1
;
Zong-Gui WU
;
Jin-Ming CHEN
;
Chao HUANG
;
Ling-Zhen ZHANG
;
Li LI
;
Ren-Qian ZHONG
Author Information
1. Changzheng Hospital Second Military Medical University
- From:
Academic Journal of Second Military Medical University
2001;22(2):127-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective： To investigate the effect of c ytokines (IFN-γ，TNF and IL-1) on the expression of CD40 and CD40 ligand (CD4 0L) in monocytes/macrophages. Methods： The mRNA expression of C D40 and CD40L was measured by RT-PCR and the CD40，CD40L expression on the mono cytes/macrophages were detected by flow cytometric analysis. Results： IFN-γ，TNF and IL-1 could not only significantly up-regulate the mRNA levels of CD40 and CD40L in cultured monocytes/macrophages, but also increase t he expression of CD40 and CD40L. Antioxidant VitE could reduce the expression o f CD40 and CD40L induced by IFN-γ，TNF and IL-1. Conclusion： IFN-γ，TNF and IL-1 can stimulate high expression of CD40 and CD40L . Antio xidant VitE can partially inhibit the expression of CD40 and CD40L induced by cy tokines in cultured monocytes/macrophages.
