Effects of oxidized low density lipoprotein and antioxidant vitamin E on the exp ression of CD40 and CD40 ligand in cultured human monocytes
10.3321/j.issn:0258-879X.2001.02.007
- VernacularTitle:VitE及oxLDL对人单核细胞表达CD40和CD40L的影响
- Author:
Zong-Gui WU
1
;
Jin-Chuan YAN
;
Ling-Zhen ZHANG
;
Li LI
;
Ren-Qian ZHONG
Author Information
1. Changzheng Hospital Second Military Medical University
- From:
Academic Journal of Second Military Medical University
2001;22(2):124-126
- CountryChina
- Language:Chinese
-
Abstract:
Objective： To investigate the effect of oxLDL and VitE on the expression of CD40 and CD40 ligand(CD40L) in cultured human monoc ytes. Methods： The expression of CD40 and CD40L on monocytes su rface were measured by indirect immunorescence technique in combination with flo w cytometry. Results： Low concentration of oxLDL（≤200 μg/L） significantly increased the expression of CD40 and CD40L in a dose and time dep endent manner. High concentration (>200 μg/L）of oxLDL markedly reduced the exp ression of CD40 and CD40L. When VitE was added, it significantly reduced the ex pression of CD40 and CD40L on monocytes surface induced by oxLDL in a dose-depe ndent manner. Conclusion：It is an important mechanism that the high expression of CD40 and CD40L induced by oxLDL may be contributed to the for mation of atherosclerosis. Antioxidan VitE can partially inhibit the high expres sion of CD40 and CD40L on monocytes surface induced by oxLDL.
